https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52589 Wed 28 Feb 2024 15:29:42 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34315 Wed 27 Feb 2019 12:25:36 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30418 Wed 11 Apr 2018 18:44:50 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17021 Wed 11 Apr 2018 17:14:33 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29178 Wed 11 Apr 2018 17:00:36 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22793 Wed 11 Apr 2018 10:52:40 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17101 Wed 11 Apr 2018 09:35:27 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34994 Thu 28 Oct 2021 13:04:29 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44697 Thu 20 Oct 2022 09:51:36 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21485 Salmonella enteritidis) or saline on postnatal days (PNDs) 3 and 5 and later subjected to the formalin test at PNDs 7, 13, and 22. One hour after formalin injection, blood was collected to assess corticosterone responses. Transverse spinal cord slices were also prepared for whole-cell patch clamp recording from lumbar superficial dorsal horn neurons (SDH). Brains were obtained at PND 22 and the hypothalamus was isolated to measure glucocorticoid (GR) and mineralocorticoid receptor (MR) transcript expression using qRT-PCR. Behavioural analyses indicate that at PND 7, no significant differences were observed between saline- or LPS-challenged rats. At PND 13, LPS-challenged rats exhibited enhanced licking (p < .01), and at PND 22, increased flinching in response to formalin injection (p < .05). LPS-challenged rats also displayed increased plasma corticosterone at PND 7 and PND 22 (p < .001) but not at PND 13 following formalin administration. Furthermore, at PND 22 neonatal LPS exposure induced decreased levels of GR mRNA and increased levels of MR mRNA in the hypothalamus. The intrinsic properties of SDH neurons were similar at PND 7 and PND 13. However, at PND 22, ipsilateral SDH neurons in LPS-challenged rats had a lower input resistance compared to their saline-challenged counterparts (p < .05). These data suggest neonatal LPS exposure produces developmentally regulated changes in formalin-induced behavioural responses, corticosterone levels, and dorsal horn neuron properties following noxious stimulation later in life. These findings highlight the importance of immune activation during the neonatal period in shaping pain sensitivity later in life. This programming involves both spinal cord neurons and the HPA axis.]]> Sat 24 Mar 2018 08:03:35 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21747 Sat 24 Mar 2018 08:03:33 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20532 Sat 24 Mar 2018 08:02:41 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19648 Sat 24 Mar 2018 08:01:08 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21584 Sat 24 Mar 2018 08:00:44 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17701 Sat 24 Mar 2018 07:57:31 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:5238 Sat 24 Mar 2018 07:44:17 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22108 Sat 24 Mar 2018 07:10:20 AEDT ]]>